
When reports began circulating in early 2025 that commercial beekeepers were experiencing higher than normal losses, the USDA moved quickly to take samples in California. Project Apis m. also conducted a survey of commercial beekeepers to better understand the scale of losses. The Domestic Losses Steering Committee was formed in mid-February and consists of the American Beekeeping Federation, the American Honey Producers Association, the Apiary Inspectors of America, the Almond Board of California, Project Apis m., the Honey Bee Health Coalition and USDA ARS. This group has developed the following Frequently Asked Questions (FAQs) that contains all known information to date. Please share these FAQs with your networks
Frequently Asked Questions
June 2025
June 2025
The following Frequently Asked Questions have been compiled from existing resources and expert perspectives. Each beekeeping operation is unique and results may vary.
Sampling and Analysis:
How many samples were taken of this event? (operations, apiaries, colonies)
Several hundred samples were collected in January and early February, 2025 from seven commercial beekeeping operations with multiple yards and geographic histories. Results for the upcoming scientific journal manuscript were generated from pooled colony and individual bee samples collected from 120 colonies in California
What were the main findings?
USDA found that high levels of Deformed wing virus A and B and acute bee paralysis are, at least in part, linked with recent colony collapses and losses across the United States. The high rates of Amitraz resistance genes in the samples “underscore the need for new parasitic treatment strategies,” according to the press release. Other analyses on pesticides and new, previously unknown diseases are ongoing to determine the roles of these in colony losses.
Which viruses and parasites were included in the testing ?
Samples were screened for nine viruses and two gut parasites (Nosema ceranae and Lotmaria passim). The viruses most tightly tied to bee death were two versions of Deformed wing virus, Deformed wing virus A and B, and Acute bee paralysis virus, all three of which are ably vectored by Varroa mites.
Was amitraz resistance tested?
A genetic trait linked with amitraz resistance was found in all collected Varroa mites. This indicates a predisposition to Amitraz resistance. It’s best to rotate among various cultural and chemical treatments to avoid the development of resistance and only use registered products. If you are interested in testing your mites for Varroa resistance, please contact Frank Rinkevich at frank.rinkevich@usda.gov, who conducts testing for research purposes. Additional laboratories offering mite resistance testing include the National Agricultural Genotyping Center, and the Florida Bee Diagnostics Lab.
Could this be pesticides? (when will we know?)
The results of virus testing have not ruled out the impact of other known bee stressors, including pesticides and poor nutrition.The pesticide analysis will be released as soon as possible.
What is still being studied?
Studies continue to refine the most important viruses for bee morbidity. Pesticide residues in samples from the same colonies are being analyzed. Additionally, beekeeper survey responses are being reviewed to assess colony losses, financial impacts, and to identify correlations with management practices and geographical factors.
How did beekeepers respond to the losses?
In addition to participating in the survey and providing samples to help understand the factors affecting losses, many beekeepers have shown exceptional resilience by working to rebuild their colonies and sustain pollination services, although at significant cost in time, labor, and resources.
How does this event differ from the losses seen in Florida two years ago?
As reported by USDA ARS (https://www.mdpi.com/2079-7737/13/2/117), numerous commercial beekeepers suffered devastating colony losses in early 2023. These losses were not as widespread as reported in 2024-2025, but nevertheless caused catastrophic harm. Samples from this event were analyzed for pathogens and pesticide incidence, with some analyses ongoing.
What do we know about the impact of colony losses to almond yields?
Almond growers have reported anecdotally that some received weaker hives than they had contracted for. There is no comprehensive data to evaluate hives delivered to growers.
The USDA National Agricultural Statistics Service (NASS) annual subjective estimate was released on May 12, 2025, projecting a 2.8 billion pound crop, which would be 3% above last year’s crop. According to the USDA, the number of bearing acres is up by 10,000 over last year. However, the previous 10-year weighted average yield is 2,096 pounds per acre. Using the Subjective Estimate of 2.8 billion pounds and the forecast 1.39 million acres produces a forecast yield of 2,014 pounds per acre, clearly a below average crop.
Yields have been down the last several years as growers worked to reduce costs and curtailed crop inputs due to poor profitability. There is also evidence in some regions that the record-breaking heat last summer may impact yields this year.
A second objective estimate will be released by NASS in July, before harvest begins.
Despite the lack of evidence to date that the high hive losses have impacted this year’s crop, the almond industry remains highly concerned about the future impacts on beekeepers and pollination services. In collaboration with honey bee organizations, the almond industry plans to invest in a new pilot with commercial beekeepers to demonstrate the efficacy of varroa treatment approaches.
Management:
Now that we know the viruses that were found, what should beekeepers do or not do?
Deformed wing virus A and B and acute bee paralysis are spread by Varroa mites and signs of Amitraz resistance genes in the mites were found in virtually all collected mite samples. Guidance on treating honey bee colonies for Varroa and avoiding the development of Amitraz resistance can be found at Hive Management – Honey Bee Health Coalition.
As in all virus containment, beekeepers should:
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Never let varroa levels get high (above a 2% infestation threshold). Monitor for mites to determine when treatments are needed and monitor after treatments to verify mite populations were reduced.
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Don’t rely on a single miticide to treat varroa.
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Move away from reliance solely on amitraz
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Rotate between different active ingredients with different modes of actions (formic acid, oxalic acid, thymol, hops beta acids)
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Combine miticide treatments with other cultural techniques (e.g. brood breaks).
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Utilize biosecurity measures.
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Clean hive tools with alcohol or flame in between yards.
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Refrain from using equipment from deadout or struggling colonies for a few weeks. This allows time for the viruses to dissipate.
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If you have colonies that are sick or struggling:
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Keep them isolated from healthy colonies.
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Minimize moving frames from sick colonies to healthy colonies.
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Healthy hives are more resilient.
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Replace old black comb with foundation regularly. Old black comb can harbor bacteria, fungal spores and pesticides which can further contribute to colony stress.
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Make sure your colonies have healthy, well-mated, young queens.
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Ensure that your colonies have adequate food stores. If they do not, provide supplemental feeding (see the HBHC’s Nutrition Guide.)
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If the causes could be pesticides, what should beekeepers do? or not do?
Results from pesticide analysis have not been reported at this time. As always, beekeepers are encouraged to use miticides according to the label instructions, develop relationships with local growers and seek notification prior to spraying.
Can we re-use our equipment?
Unknown at this time, studies suggest that equipment and tools exposed to air for several weeks have a decreased chance for harboring infectious viruses. See guidance above.
What should beekeepers do at this time to protect against these viruses?
Besides controlling mite numbers, it is known that reducing chemical stress and providing diverse pollen nutrition can reduce virus impacts. Removal of infectious material in some cases may be warranted. For example, isolating colonies with morbidities which can harbor infectious material. This is especially important before collapsed colonies get robbed out.
How can the inclusion of selected stock that maintains lower levels of Varroa be expanded?
Mite-resistant stock is likely to play a role in reducing these losses and beekeepers are encouraged to select or purchase genetic stock from reliably resistant lineages.
Services available and who prepared these FAQs:
What services are available to beekeepers?
The USDA Farm Services Agency, with offices in each county, can provide assistance to commercial beekeepers in certain cases. This includes the Emergency Assistance for Livestock, Honeybees, and Farm-Raised Fish (ELAP.)
Who is putting this guidance together?
When reports began circulating in early 2025 that commercial beekeepers were experiencing higher than normal losses, the USDA moved quickly to take samples in California. Project Apis m. also conducted a survey of commercial beekeepers to better understand the scale of losses. The Domestic Losses Steering Committee was formed in mid-February.
The Committee consists of:
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American Beekeeping Federation
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American Honey Producers Association
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Apiary Inspectors of America
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Almond Board of California
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Project Apis m.
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Honey Bee Health Coalition
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USDA ARS
Please note, if you have other questions that are not answered in this document, please let us know by emailing Honey Bee Health Coalition facilitator Matt Mulica @ mmulica@keystone.org